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响应面法优化精制沙丁鱼油EPA和DHA富集工艺
Optimization of EPA and DHA enrichment process of refined sardine oil by response surface methodology
  
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中文关键词:  精制沙丁鱼油  二十碳五烯酸（EPA）  二十二碳六烯酸（DHA）  响应面法  乙酯型
英文关键词:refined sardine oil  EPA  DHA  response surface methodology  ethyl ester type
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Author NameAffiliation
ZHANG Mengna, SONG Gongshuai, PENG Xi，etc  
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中文摘要:
      以乙酯型精制沙丁鱼油（EPA含量（15.41±0.13）%，DHA含量（8.00±0.03）%）为原料，在尿酯比、包合温度、醇尿比、包合时间4个单因素试验的基础上，采用响应面分析法优化尿素包合法富集工艺。结果表明：最优工艺条件为尿酯比3.5∶?1、包合温度0?℃、醇尿比2.6∶?1、包合时间1.2 h，此条件下，产物中EPA+DHA含量为（78.12±0.12）%（EPA含量（53.35±0.10）%；DHA含量（24.77±0.02）%），与理论值78.17%基本一致。
英文摘要:
      Using refined sardine oil ethyl ester（EPA content(15.41±0.13)%, DHA content(8.00±003)%）as raw material, the effects of urea-ester ratio, inclusion temperature, ethanol-urea ratio and inclusion time on the EPA and DHA enrichment process were studied by single factor experiment, and the response surface methodology was used to optimize the urea inclusion enrichment process. The results showed that the optimal process conditions were obtained as follows: urea-ester ratio 3.5∶?1, inclusion temperature 0?℃, ethanol- urea ratio 2.6∶?1, and inclusion time 1.2 h. Under these conditions, the total content of EPA and DHA was(78.12±0.12)%(EPA content(53.35±0.10)%, DHA content(24.77±0.02)%), which was consistent with the theoretical value of 78.17%.
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