| The fatty acid compositions of corn oil(CO), virgin grape seed oil(GSO) and lard(Lard) were determined by gas chromatography(GC). The contents of VE and polyphenols in CO and GSO were determined by high performance liquid chromatography(HPLC) and Folin-Ciocalteu. Forty-eight C57BL/6J mice were randomly divided into four groups: normal control group(ND), 45% high fat corn oil group(CO45%), 45% high fat virgin grape seed oil group(GSO45%) and 45% high fat lard group(Lard45%). The body weights of mice were measured every week. The glucose tolerance(GTT) and insulin tolerance(ITT) of mice were measured in the 12th week and the 14th week respectively. The serum levels of total cholesterol(TC), triglyceride(TG), high density lipoprotein(HDL), low density lipoprotein(LDL), free fatty acid(FFA), liver levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) inflammatory factors were measured after mice were sacrificed. The results showed that the content of polyunsaturated fatty acids in CO, GSO and Lard were 58.70%, 7415% and 13.76% respectively. The content of VE in CO was 254.06 mg/kg, which was mainly α-tocopherol and γ-tocopherol. The content of VE in GSO was 697.42 mg/kg, which was α-tocopherol, α-tocotrienol and γ-tocotrienol. Polyphenol content in GSO was 43.813 mg/kg, however, the polyphenols in CO were not detected. Animal experiments showed that compared with CO45% and Lard45%, the net body weight of mice, the serum levels of LDL and FFA, the liver levels of IL-6 and TNF-α inflammatory factors in GSO45% decreased significantly(p<0.05). Compared with Lard45%,the serum levels of TC and TG in GSO45% decreased signifcantly(p<0.05). However, there was no significant difference in serum level of HDL among different groups(p>0.05). Compared with CO45% and Lard45%, GSO45% showed better glucose tolerance and insulin resistance, which could improve insulin sensitivity. |
