| The protective effect of Gardenia oil against alcohol-induced acute hepatic injury in mice was investigated. Totally 72 mice were randomly divided into normal group, model group, low, medium and high dose group(0.3,0.6,1.2 g/kg) and positive control group(bifendate pill, 200 mg/kg), 12 mice in each group, and the normal group and the model group were treated with an equal amount of 0.5% Tween-80 solution. The acute liver injury model was induced by modified method, and the model, positive control and experimental groups were orally administrated 56% alcohol(15 mL/kg) twice at 12 h interval on the tenth day after drugs administration. After 12 h, the mice were sacrificed to contribute blood and liver for biochemical and histological examinations. The results showed that compared with the model group, serum ALT, AST, γ-GT and TG contents reduced, ADH, GSH, GSH-Px and SOD activities improved, MDA generation and the relative expression of TNF-α mRNA decreased, and liver tissue degeneration and necrosis and other pathological symptoms improved significantly in each Gardenia oil dose group especially in the high dose group. The Gardenia oil had the protective effect against alcohol-induced acute hepatic injury which might be depended on the enhancement of lipid metabolism level of liver tissue, improvement of the antioxidant enzyme system activity, inhibition of oxidative stress and reduction of inflammation factors expression. |
