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| Mutation breeding of high arachidonic acid-producing strain in Mortierella alpina |
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| DOI： |
| KeyWord:arachidonic acid Mortierella alpina mutation breeding oil yield atmospheric and room temperature plasma |
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| Abstract: |
| Obtaining the strains with good properties through breeding technologies is the precondition of achieving industrial production of microbial oil. Mortierella alpina TSM-3 was selected as the original strain, which was screened through a quick and efficient screening strategy for oleaginous filamentous fungi established by laboratory before. By the method of atmospheric and room temperature plasma (ARTP) mutagenesis, the oil and arachidonic acid(AA) yields reached 5.07 g/L and 1.55 g/L in the stable genetic mutant strain M. alpina TSM-3-1, which increased by 47.81% and 84.52% compared with the original strain. By means of combining the quick and efficient screening strategy with ARTP mutation breeding, the screening efficiency and oil producing capacity of wild strain were well enhanced, which provided a novel research strategy in the study of oleaginous filamentous fungi. |
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