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| 中性蛋白酶提取植脂末油脂工艺的研究 |
| Extraction of oil from non-dairy creamer by neutral protease |
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| DOI： |
| 中文关键词: 植脂末 中性蛋白酶 油脂提取 |
| 英文关键词:non-dairy creamer neutral protease oil extraction |
| 基金项目: |
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| 中文摘要: |
| 利用中性蛋白酶将植脂末破乳并对其酶解条件和油脂提取条件进行优化。结果表明:最佳工艺条件为25?℃、pH 7.0、中性蛋白酶加酶量6 000 U/g、酶解时间10 min、油脂提取体系为氯仿-甲醇-水（体积比2∶?2∶?1）；在最佳条件下，离心后植脂末提油率可达95.36%。中性蛋白酶能够高效破乳从而大幅提高植脂末的提油率。 |
| 英文摘要: |
| The neutral protease was used to demulsify the non-dairy creamer and the enzymolysis conditions and oil extraction conditions were optimized. The results showed that the optimal process conditions were obtained as follows: 25?℃, pH 7.0, neutral protease dosage 6 000 U/g, enzymolysis time 10 min, oil extraction system chloroform- methanol-water(volume ratio 2∶?2∶?1). Under these conditions, the oil extraction rate was up to 95.36% after centrifugation. The neutral protease could efficiently demulsify and greatly increase the oil extraction rate of non-dairy creamer. |
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