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Extraction of oil from non-dairy creamer by neutral protease |
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KeyWord:non-dairy creamer neutral protease oil extraction |
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Abstract: |
The neutral protease was used to demulsify the non-dairy creamer and the enzymolysis conditions and oil extraction conditions were optimized. The results showed that the optimal process conditions were obtained as follows: 25?℃, pH 7.0, neutral protease dosage 6 000 U/g, enzymolysis time 10 min, oil extraction system chloroform- methanol-water(volume ratio 2∶?2∶?1). Under these conditions, the oil extraction rate was up to 95.36% after centrifugation. The neutral protease could efficiently demulsify and greatly increase the oil extraction rate of non-dairy creamer. |
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