超声辅助提取葵花籽黄酮及其抗氧化活性研究
Ultrasound-assisted extraction of flavonoids from sunflower seed meal and its antioxidant activity
  
DOI:
中文关键词:  葵花籽粕  黄酮  提取  超声辅助  抗氧化活性
英文关键词:sunflower seed meal  flavonoids  extraction  ultrasound-assist  antioxidant activity
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Author NameAffiliation
ZHANG Hairong,HE Zemin,GAO Ruiyuan,etc  
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中文摘要:
      研究了超声辅助提取葵花籽黄酮的最佳工艺。通过单因素实验选取超声功率、提取温度、液料比、提取时间为考察因素,利用响应面法Box-Behnken设计对提取葵花籽黄酮工艺参数进行优化,并用DPPH·法评价葵花籽黄酮体外抗氧化活性。结果表明:超声辅助提取葵花籽黄酮的最优工艺参数为超声功率120 W、提取温度64?℃、液料比33∶?1、提取时间28 min,在此条件下,葵花籽黄酮得率为1.91%;葵花籽黄酮对DPPH·清除率IC50为0.09 mg/mL,优于食品抗氧化剂BHT对DPPH·清除率(IC50为0.11 mg/mL);与传统的索氏提取法对比,超声辅助法提取葵花籽黄酮时间短、节能和得率高。
英文摘要:
      The optimal technology of ultrasound-assisted extraction of sunflower seed flavonoids was studied. The ultrasonic power, extraction temperature, liquid-material ratio and extraction time were selected as factors by single factor experiment, and the process parameters of extraction of flavonoids from sunflower seed meal were optimized by response surface methodology with Box-Behnken design. The results showed that the optimal extraction parameters of flavonoids from sunflower seed meal were obtained as follows: ultrasonic power 120 W, extraction temperature 64?℃, liquid-material ratio 33∶?1 and extraction time 28 min. Under these conditions, the sunflower seed flavonoids yield was 1.91%. The IC50 of sunflower seed flavonoids on DPPH· was 0.09 mg/mL, which was better than that of food antioxidant BHT(the IC50 was 0.11 mg/mL). Compared with the traditional Soxhlet extraction method, ultrasound-assisted extraction of sunflower seed flavonoids had the advantages of short time, energy saving and high yield.
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