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| Preparation of melamine immobilized silver ion chromatography stationary phase and its application in the detection of oils and fats |
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| KeyWord:melamine silver ion chromatography stationary phase unsaturated fatty acid |
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| Abstract: |
| A novel melamine-bonded silica gel chromatography stationary phase(MBSP) was prepared using isocyanatopropyltriethoxysilane as the coupling agent and melamine as the ligand, and then the synthesized MBSP was further utilized for immobilization of silver ion according to the complexation of melamine and silver ion to prepare melamine immobilized silver ion chromatography stationary phase(AgMBSP). The AgMBSP was characterized by FTIR and elemental analysis,and the results showed that the bonding amount of melamine was 0.11 mmol/g and the loading amount of silver ion was determined as 0.17 g/g by spectrophotometry. Under the HPLC conditions of acetonitrile - n-hexane(0.3∶?99.7) and flow rate 0.5 mL/min, five kinds of unsaturated fatty acid methyl esters could be completely separated by AgMBSP. Also good results were obtained when the column was applied in the determination of unsaturated fatty acids in four common oils and fats. |
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