| The oil was extracted from hippoglossus by enzyme method, and the extraction conditions and qualities of hippoglossus oil were analyzed. With the extraction rate of hippoglossus oil as index，the extraction conditions were optimized by response surface methodology on the basis of single factor experiment. The results showed that the optimal extraction conditions were obtained as follows:mass ratio of solid to liquid 1∶?2，dosage of enzyme 0.25%(based on the mass of total material)，mass ratio of animal protease to trypsin 1∶?1.5，pH 7.5，enzymolysis temperature 52?℃and enzymolysis time 2 h. Under the optimal extraction conditions，the extraction rate of hippoglossus oil reached 79.85%. The acid value, peroxide value, insoluble impurities, moisture and volatile matter met the standard of first grade crude oil in SC/T 3502—2016. The content of saturated fatty acids in hippoglossus oil was 67.26%, while the content of unsaturated fatty acids was 33.76%, in which the total content of EPA and DHA was 13.62%. The sensory properties of crude hippoglossus oil met the standard of refined fish oil in SC/T 3502—2016. So hippoglossus oil could be regarded as a kind of new fish oil resource with high application value and development potential. |
