陕西茵陈黄酮提取及抗氧化性能研究
Extraction and antioxidant activity of flavonoids from Capillary artemisia in Shaanxi
  
DOI:
中文关键词:  茵陈  黄酮  提取  抗氧化  稳定性
英文关键词:Capillary artemisia  flavonoids  extraction  antioxidation  stability
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Author NameAffiliation
SHI Juan,ZHANG Zehao, LI Jiang,etc  
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中文摘要:
      以陕西茵陈为原料,采用超声波预处理-乙醇回流法提取菌陈黄酮,以乙醇体积分数、回流时间、回流温度和料液比为因素,茵陈黄酮得率为考察指标,确定茵陈黄酮的最佳提取工艺。研究了茵陈黄酮抗脂质过氧化能力和DPPH自由基清除能力,及温度、光照、pH、金属离子对茵陈黄酮抗氧化性能的影响。结果表明:茵陈黄酮最佳提取条件为乙醇体积分数50%、回流时间50 min、回流温度90?℃、料液比1∶?25,在此条件下茵陈黄酮得率为2.35%。温度和Na+、K+、Ca2+、Cu2+对茵陈黄酮抗氧化能力影响不大;Cd3+、Cr3+、Al3+以及pH、光照对茵陈黄酮抗氧化能力影响显著。此外,茵陈黄酮对菜籽油具有一定的抗氧化能力,分别与VC和柠檬酸协同作用时其抗氧化能力增强。
英文摘要:
      Taking Capillary artemisia in Shaanxi as raw material, with ethanol volume fraction, reflux time, reflux temperature and solid-liquid ratio as factors, and flavonoids yield as index, the extraction of flavonoids from Capillary artemisia was studied by ultrasonic pretreatment-ethanol reflux method. The antioxidant activity of Capillary artemisia flavonoids was determined by studying the anti-lipid peroxidation ability and scavenging DPPH free radical ability. The results showed that the optimal extraction conditions of flavonoids from Capillary artemisia were obtained as follows: ethanol volume fraction 50%, reflux time 50 min, reflux temperature 90?℃ and solid-liquid ratio 1∶?25. Under these conditions, flavonoids yield was 2.35%. Temperature, Na+, K+, Ca2+ and Cu2+ had little effect on the antioxidant ability of Capillary artemisia flavonoids. Light illumination, pH, Cd3+, Cr3+ and Al3+ had significant effect on the antioxidant ability of Capillary artemisia flavonoids. In addition, Capillary artemisia flavonoids had certain antioxidant capacity on rapeseed oil, and vitamin C and citric acid exhibited a synergistic antioxidation effect.
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