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| 酸预处理麦秆半同步和同步糖化发酵制乙醇条件优化 |
| Optimization of semi-simultaneous and simultaneous saccharification and fermentation conditions of acid pretreated wheat straw to produce ethanol |
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| DOI： |
| 中文关键词: 麦秆 盐酸预处理 半同步糖化发酵 同步糖化发酵 正交实验 XRD |
| 英文关键词:wheat straw hydrochloric acid pretreatment semi-simultaneous saccharification and fermentation simultaneous saccharification and fermentation orthogonal experiment XRD |
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| 中文摘要: |
| 麦秆首先进行盐酸预处理，然后以盐酸预处理麦秆为底物通过正交实验优化了底物半同步和同步糖化发酵制乙醇条件。利用XRD对原料、酸预处理麦秆和发酵麦秆的结构特征进行分析。结果表明:盐酸预处理的麦秆半同步糖化发酵制乙醇的最佳条件为发酵温度36?℃、酵母接种量01%、酶质量浓度0.8 g/L和发酵时间2 d，此时乙醇含量为19.16 g/L；盐酸预处理的麦秆同步糖化发酵制乙醇的最佳条件为发酵温度39?℃、酵母接种量0.1%、酶质量浓度0.5 g/L和发酵时间4 d，此时乙醇含量为19.44 g/L；同步糖化发酵优于半同步糖化发酵；XRD分析表明酸预处理和发酵后，麦秆的结晶度降低。 |
| 英文摘要: |
| Wheat straw was first pretreated by hydrochloric acid, and then the semi-simultaneous and simultaneous saccharification and fermentation conditions to produce ethanol were optimized by orthogonal experiment using hydrochloric acid pretreated wheat straw as substrate. XRD was used to analyze the structural characteristics of raw material, acid pretreated wheat straw and fermented wheat straw. The results showed that the optimal semi-simultaneous saccharification and fermentation conditions of hydrochloric acid pretreated wheat straw to produce ethanol were obtained as follows: fermentation temperature 36?℃, yeast inoculation amount 0.1%, enzyme mass concentration 0.8 g/L and fermentation time 2 d. Under these conditions, the ethanol content was 19.16 g/L. The optimal simultaneous saccharification and fermentation conditions of hydrochloric acid pretreated wheat straw to produce ethanol were obtained as follows: fermentation temperature 39?℃, yeast inoculation amount 0.1%, enzyme mass concentration 0.5 g/L and fermentation time 4 d. Under these conditions, the ethanol content was 19.44 g/L. Simultaneous saccharification and fermentation was superior to semi-simultaneous saccharification and fermentation. XRD analysis showed that the crystallinity of acid pretreated wheat straw and fermented wheat straw reduced. |
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