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| Optimization of semi-simultaneous and simultaneous saccharification and fermentation conditions of acid pretreated wheat straw to produce ethanol |
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| DOI： |
| KeyWord:wheat straw hydrochloric acid pretreatment semi-simultaneous saccharification and fermentation simultaneous saccharification and fermentation orthogonal experiment XRD |
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| Abstract: |
| Wheat straw was first pretreated by hydrochloric acid, and then the semi-simultaneous and simultaneous saccharification and fermentation conditions to produce ethanol were optimized by orthogonal experiment using hydrochloric acid pretreated wheat straw as substrate. XRD was used to analyze the structural characteristics of raw material, acid pretreated wheat straw and fermented wheat straw. The results showed that the optimal semi-simultaneous saccharification and fermentation conditions of hydrochloric acid pretreated wheat straw to produce ethanol were obtained as follows: fermentation temperature 36?℃, yeast inoculation amount 0.1%, enzyme mass concentration 0.8 g/L and fermentation time 2 d. Under these conditions, the ethanol content was 19.16 g/L. The optimal simultaneous saccharification and fermentation conditions of hydrochloric acid pretreated wheat straw to produce ethanol were obtained as follows: fermentation temperature 39?℃, yeast inoculation amount 0.1%, enzyme mass concentration 0.5 g/L and fermentation time 4 d. Under these conditions, the ethanol content was 19.44 g/L. Simultaneous saccharification and fermentation was superior to semi-simultaneous saccharification and fermentation. XRD analysis showed that the crystallinity of acid pretreated wheat straw and fermented wheat straw reduced. |
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