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| 地参总多酚提取及其抗氧化活性研究 |
| Extraction and antioxidant activity of total polyphenol from Lycopus lucidus Turcz. |
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| DOI： |
| 中文关键词: 地参 多酚 响应面法 提取 抗氧化活性 |
| 英文关键词:Lycopus lucidus Turcz. polyphenol response surface methodology extraction antioxidant activity |
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| 中文摘要: |
| 以总多酚得率为考察指标，用乙醇冷凝回流法，在单因素试验基础上，响应面优化制备地参总多酚，测定其抗氧化活性。结果表明：地参总多酚提取最佳工艺条件为料液比1∶?58、乙醇体积分数60%、提取温度90?℃、提取时间3 h，在此条件下地参总多酚得率为(1.08±0.56)%；地参总多酚清除DPPH·的IC50为114 μg/mL。表明响应面优化的地参总多酚提取工艺稳定可行，地参总多酚具有一定的抗氧化活性。 |
| 英文摘要: |
| With yield of total polyphenol as investigation indicator, the preparation process of total polyphenol from Lycopus lucidus Turcz. by ethanol condensation reflux method was optimized by response surface methodology based on single factor experiment. The antioxidant activity of total polyphenol was determined. The results showed that the optimal extraction process conditions were obtained as follows: ratio of material to liquid 1∶?58, volume fraction of ethanol 60%, extraction temperature 90?℃ and extraction time 3 h. Under these conditions, the yield of total polyphenol was (1.08±0.56)%. The IC50 of scavenging DPPH free radical by total polyphenol was 114 μg/mL. The extraction process of total polyphenol from Lycopus lucidus Turcz. optimized by response surface methodology was stable and feasible, and the total polyphenol from Lycopus lucidus Turcz. had a certain antioxidant activity. |
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