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| Extraction and antioxidant activity of total polyphenol from Lycopus lucidus Turcz. |
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| DOI： |
| KeyWord:Lycopus lucidus Turcz. polyphenol response surface methodology extraction antioxidant activity |
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| Abstract: |
| With yield of total polyphenol as investigation indicator, the preparation process of total polyphenol from Lycopus lucidus Turcz. by ethanol condensation reflux method was optimized by response surface methodology based on single factor experiment. The antioxidant activity of total polyphenol was determined. The results showed that the optimal extraction process conditions were obtained as follows: ratio of material to liquid 1∶?58, volume fraction of ethanol 60%, extraction temperature 90?℃ and extraction time 3 h. Under these conditions, the yield of total polyphenol was (1.08±0.56)%. The IC50 of scavenging DPPH free radical by total polyphenol was 114 μg/mL. The extraction process of total polyphenol from Lycopus lucidus Turcz. optimized by response surface methodology was stable and feasible, and the total polyphenol from Lycopus lucidus Turcz. had a certain antioxidant activity. |
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