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Heterologous expression and activity of the genes related to CLA synthesis in Yarrowia lipolytica |
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DOI: |
KeyWord:linoleic acid conjugated linoleic acid Yarrowia lipolytica heterologous expression |
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Abstract: |
To explore whether three genes involved in conjugated linoleic acid (CLA) biosynthesis in Lactobacillus plantarum(Myosin cross reactive antigen(mcra), Short-chain dehydrogenase/oxidoreductase(dh) and Acetoacetate decarboxylase(dc)) were active after heterologously expressed in Yarrowia lipolytica (Y. lipolytica), with two Y. lipolytica expression plasmids (pINA 1269 and pINA 1312), the three genes were introduced into Y. lipolytica auxotrophic host Polf (Ura-, Leu-), and finally the recombinant strains were constructed. The corresponding substrates linoleic acid (LA) and 10-hydroxy-cis-12-octadecenoic acid (10-HOE) were added into different recombinant strains, and the fatty acids were detected in the reaction system to obtain different products corresponding to the genes: 10-HOE and 10-oxo-trans 11-octadecenoic acid, demonstrating that mcra, dh and dc were heterologously expressed and were active in Y. lipolytica. |
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