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| Effects of three different culture mediums on growth and lipid accumulation of Scenedesmus obliquus |
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| KeyWord:Scenedesmus obliquus culture medium growth level lipid fatty acid |
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| Abstract: |
| Three kinds of culture mediums (BG11, F/2 and SE) were used to culture Scenedesmus obliquus FACHB-12. The effects of different culture mediums on growth and lipid accumulation of Scenedesmus obliquus were investigated by determining its biomass, growth rate, lipid content, lipid fraction distribution and fatty acid composition. The results showed that BG11 medium was more conducive to the growth of Scenedesmus obliquus, while the F/2 medium was more suitable for the lipid and neutral lipid accumulation. The specific growth rate, biomass and chlorophyll a content of Scenedesmus obliquus were respectively 0.095, 0.36 g/L and 1.23 mg/L after being cultured for 15 d in BG11 medium. The lipid content of Scenedesmus obliquus reached 24.75% of dry cell weight in Scenedesmus obliquusin F/2 medium, in which the neutral lipid accounted for 36.64% and its productivity was 0.98 mg/(L·d). In addition, the C16∶?0 and C18∶?1 was main fatty acids in Scenedesmus obliquusin in F/2 medium, which accounted for 25.84% and 54.47% of the total fatty acids respectively. Therefore, it was an ideal raw material for biodiesel. |
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