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| Effects of medium- and long-chain fatty acids on apoptosis and lipogenesis of 3T3-L1 adipocyte |
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| KeyWord:medium- and long-chain fatty acid pre-adipocyte apoptosis lipogenesis |
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| Abstract: |
| To investigate the effects of different types and concentrations of free fatty acids on apoptosis and lipogenesis of 3T3-L1 adipocyte, 3T3-L1 adipocyte was treated by different types and concentrations of medium- and long- chain fatty acids to observe the apoptosis and lipogenesis of the cell. The results indicated that medium- and long-chain fatty acids inhibited the proliferation of 3T3-L1 cells in a dose-dependent manner. C16∶?0, C18∶?0, C18∶?3c6,9,12 and EPA showed significantly different inhibitory effect on cell proliferation compared with the control group, particularly DHA showed dramatical roles on inhibition of cell proliferation. Moreover, C18∶?3c6,9,12 and EPA significantly increased the production of cleaved PARP protein, indicating that C18∶?3c6,9,12 and EPA could induce cell apoptosis.Oil red staining results showed that the EPA and DHA at the concentrations of 150 μmol/L and 180 μmol/L significantly enhanced lipogenesis of adipocyte. In conclusion, different fatty acids had different effects on cell growth, proliferation and apoptosis, in particular,EPA could significantly induce apoptosis and EPA and DHA could promote lipogenesis of 3T3-L1 adipocyte. |
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