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| 中长链脂肪酸对3T3-L1脂肪细胞凋亡和脂肪生成的作用 |
| Effects of medium- and long-chain fatty acids on apoptosis and lipogenesis of 3T3-L1 adipocyte |
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| DOI： |
| 中文关键词: 中长链脂肪酸 前脂肪细胞 细胞凋亡 脂肪生成 |
| 英文关键词:medium- and long-chain fatty acid pre-adipocyte apoptosis lipogenesis |
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| 中文摘要: |
| 为了探究不同种类、不同浓度脂肪酸对3T3-L1脂肪细胞凋亡和脂肪生成的影响，以3T3-L1脂肪细胞为研究对象，用不同种类、不同浓度的中长链脂肪酸处理细胞，观察细胞凋亡和脂肪生成情况。结果发现：中长链脂肪酸可以抑制3T3-L1脂肪细胞增殖，并且存在剂量依赖性，C16∶?0、C18∶?0、C18∶?3c6,9,12、EPA处理组与对照组相比，具有显著性差异，而DHA处理组与对照组相比具有极显著性差异；C18∶?3c6,9,12和EPA处理后，剪切形式PARP增加，说明C18∶?3c6,9,12和EPA能诱导细胞凋亡；油红O染色结果表明两种浓度（150 μmol/L和180 μmol/L）的EPA和DHA均能显著促进脂肪细胞的脂肪生成。综上可知，不同脂肪酸对细胞生长、增殖和凋亡有不同作用，特别是EPA能诱导细胞凋亡，EPA和DHA可显著促进细胞的脂肪生成。 |
| 英文摘要: |
| To investigate the effects of different types and concentrations of free fatty acids on apoptosis and lipogenesis of 3T3-L1 adipocyte, 3T3-L1 adipocyte was treated by different types and concentrations of medium- and long- chain fatty acids to observe the apoptosis and lipogenesis of the cell. The results indicated that medium- and long-chain fatty acids inhibited the proliferation of 3T3-L1 cells in a dose-dependent manner. C16∶?0, C18∶?0, C18∶?3c6,9,12 and EPA showed significantly different inhibitory effect on cell proliferation compared with the control group, particularly DHA showed dramatical roles on inhibition of cell proliferation. Moreover, C18∶?3c6,9,12 and EPA significantly increased the production of cleaved PARP protein, indicating that C18∶?3c6,9,12 and EPA could induce cell apoptosis.Oil red staining results showed that the EPA and DHA at the concentrations of 150 μmol/L and 180 μmol/L significantly enhanced lipogenesis of adipocyte. In conclusion, different fatty acids had different effects on cell growth, proliferation and apoptosis, in particular,EPA could significantly induce apoptosis and EPA and DHA could promote lipogenesis of 3T3-L1 adipocyte. |
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