With leaching crude sesame oil as raw material and ethanol as extractant, sesame lignan was extracted. The optimal extraction conditions of sesamin were obtained by single factor experiment and orthogonal experiment. The contents of sesamin and sesamolin in crude sesame lignan were determined by HPLC. The results showed that the optimal extraction conditions were obtained as follows: leaching times three, solid-liquid ratio 1∶?4, extraction temperature 60?℃, volume fraction of ethanol 95%, stirring time 0.5 h, standing time 2 h and stirring speed 400 r/min. Under these conditions, the extraction rate of sesamin was 86.99%, and the contents of sesamin and sesamolin in crude sesame lignan were 4873% and 5.71% respectively. |