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| Rapid simultaneous determination of vitamin E, phytosterol and squalene in virgin crude grape seed oil |
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| DOI： |
| KeyWord:virgin crude grape seed oil phytos terol stanol squalene tocotrienol tocopherol |
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| Abstract: |
| An accurate and fast method was established to detect vitamin E, phytosterol(campesterol,stigmasterol,β-sitosterol, stanol) and squalene content in virgin crude grape seed oil by HPLC. Sample was saponificated at high temperature, then extracted by absolute ethyl ether, purified and detected by HPLC. HPLC conditions were obtained as follows: mobile phase A methanol-acetonitrile-TBME(volume ratio 85.5∶?8∶?6.5),mobile phase B water-acetic acid(volume ratio 99∶?1), injection volume 20 μL, detection wavelength 292 nm for vitamin E, 210 nm for campesterol, stigmasterol and β-sitosterol and squalene, column temperature 25 ?℃, ELSD for stanol and operation time 25 min. The results showed that the mass concentrations and peark areas of eleven target objects had a good linear relationship (R2＞0.999). The detection limit was 0.10-5.00 μg/mL, and the quantification limit was 0.17-6.25 μg/mL. The vitamin E content in the sample was 357.09-655.06 mg/kg, the phytosterol content was 1 220.11-3 178.76 mg/kg, and the squalene content was 24.21-67.98 mg/kg. The method had the items of low RSD and accurate result, and could be used to detect virgin crude grape seed oil in batch. |
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