| In order to investigate in vitro antioxidant activity of Chinese giant salamander oil, the antioxidant activity of Chinese giant salamander oil was evaluated by DPPH radical, hydroxyl radical, superoxide anion radical scavenging ability assays, reducing capacity assay and capacity of chelating Fe2+ with vitamin C as positive control. The results indicated that the antioxidant ability of Chinese giant salamander oil was stronger than mackerel oil, and the antioxidant ability was stronger after adding compound antioxidant. Chinese giant salamander oil had the strongest capacity of chelating Fe2+, stronger than vitamin C under the same mass concentration condition, while it was comparable to vitamin C on scavenging ability of DPPH radical, hydroxyl radical and reducing capacity. The IC50 values of Chinese giant salamander oil on DPPH radical, hydroxyl radical and capacity of chelating Fe2+ were 3.523,0.493 mg/mL and 0.559 mg/mL respectively, and they were 0.572,0.099 mg/mL and 0.062 mg/mL for Chinese giant salamander oil added with compound antioxidant, respectively. |
