| The hypocholesterolemic peptides from broad bean protein by enzymatic hydrolysis were investigated. In order to obtain broad bean peptides with high cholesterol-lowering activity，six proteinases were compared on the degree of hydrolysis (DH) of broad bean protein and the hypocholesterolemic abilitity, and alkaline protease was employed. Then the DH and hypocholesterolemic ability were used as indicators, single factor experiment and response surface methodology were applied to get the optimal enzymatic hydrolysis parameters of broad bean protein.The results showed that the optimal enzymatic hydrolysis parameters for alkaline protease were obtained as follows: enzymolysis temperature 40?℃, pH 9.0, enzymolysis time 60 min, dosage of enzyme 8 000 U/g and ratio of material to liquid 1∶?20. Under the optimal conditions, broad bean peptides with 1429% DH could be obtained, and its inhibition rates on sodium cholate, sodium glycine and sodium taurocholate were predicted 52.49%, 31.44% and 24.76%, and the validation tests gave 52.98%, 31.87% and 25.17%, respectively. |
