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| Determination of benzopyrene in vegetable oil by liquid-phase extraction- RP-HPLC |
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| KeyWord:benzopyrene RP-HPLC liquid-phase extraction |
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| Abstract: |
| A method for determination of benzopyrene(BaP) in vegetable oil by liquid-phase extraction-reversed phase high performance liquid chromatography （RP-HPLC）was established. With n-hexane saturated by acetonitrile-acetonitrile saturated by n-hexane as extraction solvent, the content of BaP in vegetable oil was determined by RP-HPLC.The results showed that there was linear relationship between peak area and BaP mass concentration in the range of 2-100 ng/mL, the linear equation was Y=0717 823X+0.159 747，and the correlation coefficient was 0.999 99.When the volume ratio of n-hexane saturated by acetonitrile to acetonitrile saturated by n-hexane was 2∶?5 (4 mL n-hexane saturated by acetonitrile, 10 mL acetonitrile saturated by n-hexane), the recovery rate was 82.62%, the precision was 1.1%, and the limit of detection was 0.4 μg/kg. The method was stable，efficient and low cost，and was suitable for the determination of BaP in vegetable oil． |
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