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液相萃取-反相高效液相色谱法联用测定植物油中的苯并芘 |
Determination of benzopyrene in vegetable oil by liquid-phase extraction- RP-HPLC |
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DOI: |
中文关键词: 苯并芘 反相高效液相色谱 液相萃取 |
英文关键词:benzopyrene RP-HPLC liquid-phase extraction |
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中文摘要: |
建立液相萃取-反相高效液相色谱法测定植物油中苯并芘的方法,以乙腈饱和的正己烷-正己烷饱和的乙腈作为萃取试剂,采用反相高效液相色谱仪对植物油中苯并芘的含量进行测定。所得线性方程Y=0.717 823X+0.159 747,相关系数r=0.999 99,线性范围在2~100 ng/mL,当乙腈饱和的正己烷与正己烷饱和的乙腈体积比为2∶?5时(即4 mL乙腈饱和的正己烷,10 mL正己烷饱和的乙腈),其回收率在82.62%,精密度为1.1%,检出限为0.4 μg/kg。该方法稳定、高效、检测成本低,适用于植物油中苯并芘的检测。 |
英文摘要: |
A method for determination of benzopyrene(BaP) in vegetable oil by liquid-phase extraction-reversed phase high performance liquid chromatography (RP-HPLC)was established. With n-hexane saturated by acetonitrile-acetonitrile saturated by n-hexane as extraction solvent, the content of BaP in vegetable oil was determined by RP-HPLC.The results showed that there was linear relationship between peak area and BaP mass concentration in the range of 2-100 ng/mL, the linear equation was Y=0717 823X+0.159 747,and the correlation coefficient was 0.999 99.When the volume ratio of n-hexane saturated by acetonitrile to acetonitrile saturated by n-hexane was 2∶?5 (4 mL n-hexane saturated by acetonitrile, 10 mL acetonitrile saturated by n-hexane), the recovery rate was 82.62%, the precision was 1.1%, and the limit of detection was 0.4 μg/kg. The method was stable,efficient and low cost,and was suitable for the determination of BaP in vegetable oil. |
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