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| Effects of SSADH gene regulation on lipid synthesis of the oleaginous fungus Mortierella alpina |
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| KeyWord:M. alpina GABA metabolism pathway SSADH gene regulation NADPH lipogenesis |
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| Abstract: |
| M. alpina has strong ability to synthesize lipid and can synthesize a variety of n-3 and n-6 series polyunsaturated fatty acids (PUFAs). γ-Aminobutyric acid（GABA）metabolism pathway is a process in which glutamate is converted to succinic acid through a series of reactions and produces NADPH,and can provide nitrogen source and carbon source for the growth of fungi for lipid synthesis. By constructing RNA recombinant strain (MA-iSSADH ) of succinic semialdehyde dehydrogenase(SSADH) gene in GABA metabolism pathway, the fatty acid composition of MA-iSSADH, the content of NADPH and the transcriptional level of related genes generating NADPH were analyzed to investigate the role of SSADH in M. alpina during lipid synthesis. Compared with M. alpina, the transcriptional level of SSADH decreased significantly and the content of total fatty acid decreased by 20.9%,while the relative content of C18∶?1 increased by 73.5% in MA-iSSADH. It indicated that the SSADH played an important role in the lipid synthesis of M. alpina. Although there was no obvious change in content of NADPH, the transcriptional level of related genes generating NADPH such as malic acid (ME), glucose-6-phosphate dehydrogenase (G6PD), 6-phosphog luconate dehydrogenase (6PGD), isocitrate hydrogenase (IDH) and methylenetetrahydrofolate dehydrogenase (MTHFD) changed significantly,which showed that SSADH regulated the source of NADPH required for the lipogenesis, and thus played an important role in lipid synthesis of M. alpina. |
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