| Phospholipid (PL) composition in human milk from different lactation periods and gestational ages was analyzed using 31P-NMR. The results indicated that in preterm and full term human milk, the main PL were SM, PC, PE, EPLAS, PI and PS,in which the content of SM was the highest, followed by PC and PE, and the contents of PI and PS were the lowest. The content of total PL did not show significant difference in human milk from different lactation periods and gestational ages. In phospholipid composition, the contents of PI in colostrum and transitional milk in full term human milk were(4.14±042)% and (3.66±0.66)% respectively, which were significantly higher than (2.79±0.09)% of the mature milk.In preterm human milk, the contents of PC in colostrum and transitional milk were (3074±2.03)% and (29.40±2.37)% respectively, which were significantly higher than (27.55±2.42)% of the mature milk. In addition, the EPLAS content decreased and PE content increased gradually with the lactation period prolonging, while the content of PE+EPLAS had no significant difference. Moreover, there was no significant difference in milk fat globule between preterm and full term human milk. Milk fat globule membrane composed of phospholipid enveloped milk fat globule with volume mean diameter about 5 μm. |
