| The determination of short-chain fatty acid by ion chromatography and high-performance liquid chromatography(HPLC) was compared from the aspects of separation chromatography, analysis time, linearity range, limit of detection, precision and recovery. HPLC method used SunFireTM C18 chromatography column, mobile phase was KH2PO4 solution and methanol, and flow rate was 1.0 mL/min. Ion chromatography method used DionexTM ATC-3 chromatography column, mobile phase was sodium hydrate solution with different concentrations, and flow rate was 1.5 mL/min. The results showed that analysis time, linearity range, limit of detection, precisions of retention time and peak area, and recovery of HPLC method were 14.5 min, 0-1 200 mg/L, 15.57-23.23 mg/L, 0.079%-0.597%, 1.12%-3.50% and 96.1%-112.0% respectively. For ion chromatography method, they were 13.0 min, 0-20 mg/L, 0.03-0.10 mg/L, 0.000%-0.053%, 1.18%-2.69% and 98.2%-122.0%, respectively. HPLC method had wide detection range, but higher limit of detection. Ion chromatography method had lower limit of detection, but narrow detection range, and higher sampling mass concentration led to poor separation effect. So, the proper detection method should be chosen according to the actual mass concentration of sample. |
