Optimization of extraction of wild grape seed oil by acid-heating method and its fatty acid composition
  
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KeyWord:wild grape seed oil  acid-heating method  process optimization  oil extraction rate  fatty acid
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LV Huiwei, GUO Hang, WU Qiuyan,etc  
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Abstract:
      The extraction process of wild grape seed oil by acid-heating method was optimized. With the oil extraction rate as the evaluation index, the influences of hydrochloric acid concentration, ratio of solid to liquid, acid-heating time, acid-heating temperature and extraction time on the extraction effect of wild grape seed oil were studied by single factor experiment, and the process conditions were optimized by response surface methodology. The results showed that the optimal conditions were obtained as follows: hydrochloric acid concentration 4.09 mol/L, ratio of solid to liquid 1∶?6, acid-heating time 59.37 min, acid-heating temperature 75.85?℃ and extraction time 1.99 h. Under the optimal conditions, the oil extraction rate was 26.005%. The wild grape seed oil was mainly composed of linoleic acid, oleic acid, palmitic acid and stearic acid ,and the corresponding contents were 70.59%, 13.34%, 8.63% and 4.27%,respectively, while the contents of arachidic acid, linolenic acid, palmitoleic acid and myristic acid were lower.
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