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| 玉米秸秆水解液综合利用生产γ-聚谷氨酸 |
| Integrated utilization of corn stover for poly (γ-glutamic acid) production |
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| DOI： |
| 中文关键词: 玉米秸秆水解液 葡萄糖 木糖 发酵 γ-聚谷氨酸 |
| 英文关键词:corn stover hydrolysate glucose xylose fermentation poly(γ-glutamic acid) |
| 基金项目: |
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| 中文摘要: |
| 以玉米秸秆预处理以及酶水解得到的还原糖作为碳源发酵产γ-聚谷氨酸(γ-PGA), 分别探究了葡萄糖、木糖、L-谷氨酸钠一水合物和金属离子对B. subtilis CGMCC 1250生长以及γ-PGA生产的影响，在摇瓶中优化培养基组分，并进行发酵罐放大操作。结果表明：玉米秸秆经过稀碱预处理以及复合酶水解后，得到的混合糖质量浓度为（76.3±5.7）g/L，其主要成分是葡萄糖和木糖，两者比例为2.19∶?1;在配制发酵培养基时添加40 g/L的L-谷氨酸钠一水合物，及ZnSO4·7H2O 0.29 g/L、MnSO4·7H2O 0.05g/L、FeCl3·6H2O 0.11 g/L，摇瓶发酵可得到产量为（20.5±270）g/L的 γ-PGA ; 在3 L发酵罐实验中采用补料分批发酵的方式生产可以提高产物产量，得到产量为25.6 g/L的γ-PGA。 |
| 英文摘要: |
| Corn stover was pretreated and enzymatically hydrolyzed into reducing sugar to produce polyγ-glutamic acid (γ-PGA) by fermentation. The effects of glucose, xylose, L-monosodium glutamate monohydrate and metal ion on the growth of B. subtilis CGMCC 1250 and γ-PGA production were studied, and the components of the fermentation medium were optimized and fermentation was conducted in a fermenter. The results showed that corn stover was pretreated by dilute base and enzymatically hydrolyzed by compound enzyme, and mass concentration of the reducing sugar was (76.3±5.7)g/L. The hydrolysate was mainly composed by glucose and xylose, with a ratio of 2.19∶?1. Then 40 g/L of L-monosodium glutamate monohydrate, 0.29 g/L of ZnSO4·7H2O, 0.05g/L of MnSO4·7H2O and 0.11 g/L of FeCl3·6H2O were added into the initial fermentation medium, resulting into (20.5±2.70)g/L of γ-PGA. Finally, a fed-batch strategy was conducted in a 3 L fermenter to increase product yield, and 256 g/L of γ-PGA was achieved. |
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