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玉米秸秆水解液综合利用生产γ-聚谷氨酸 |
Integrated utilization of corn stover for poly (γ-glutamic acid) production |
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DOI: |
中文关键词: 玉米秸秆水解液 葡萄糖 木糖 发酵 γ-聚谷氨酸 |
英文关键词:corn stover hydrolysate glucose xylose fermentation poly(γ-glutamic acid) |
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中文摘要: |
以玉米秸秆预处理以及酶水解得到的还原糖作为碳源发酵产γ-聚谷氨酸(γ-PGA), 分别探究了葡萄糖、木糖、L-谷氨酸钠一水合物和金属离子对B. subtilis CGMCC 1250生长以及γ-PGA生产的影响,在摇瓶中优化培养基组分,并进行发酵罐放大操作。结果表明:玉米秸秆经过稀碱预处理以及复合酶水解后,得到的混合糖质量浓度为(76.3±5.7)g/L,其主要成分是葡萄糖和木糖,两者比例为2.19∶?1;在配制发酵培养基时添加40 g/L的L-谷氨酸钠一水合物,及ZnSO4·7H2O 0.29 g/L、MnSO4·7H2O 0.05g/L、FeCl3·6H2O 0.11 g/L,摇瓶发酵可得到产量为(20.5±270)g/L的 γ-PGA ; 在3 L发酵罐实验中采用补料分批发酵的方式生产可以提高产物产量,得到产量为25.6 g/L的γ-PGA。 |
英文摘要: |
Corn stover was pretreated and enzymatically hydrolyzed into reducing sugar to produce polyγ-glutamic acid (γ-PGA) by fermentation. The effects of glucose, xylose, L-monosodium glutamate monohydrate and metal ion on the growth of B. subtilis CGMCC 1250 and γ-PGA production were studied, and the components of the fermentation medium were optimized and fermentation was conducted in a fermenter. The results showed that corn stover was pretreated by dilute base and enzymatically hydrolyzed by compound enzyme, and mass concentration of the reducing sugar was (76.3±5.7)g/L. The hydrolysate was mainly composed by glucose and xylose, with a ratio of 2.19∶?1. Then 40 g/L of L-monosodium glutamate monohydrate, 0.29 g/L of ZnSO4·7H2O, 0.05g/L of MnSO4·7H2O and 0.11 g/L of FeCl3·6H2O were added into the initial fermentation medium, resulting into (20.5±2.70)g/L of γ-PGA. Finally, a fed-batch strategy was conducted in a 3 L fermenter to increase product yield, and 256 g/L of γ-PGA was achieved. |
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