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| 聚苯乙烯微球固定化海洋脂肪酶YS2071及其性能研究 |
| Polystyrene microspheres immobilizing marine lipase YS2071 and its properties |
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| DOI： |
| 中文关键词: 聚苯乙烯微球 吸附法 固定化 脂肪酶 酶活回收率 |
| 英文关键词:polystyrene microsphere adsorption method immobilization lipase enzyme recovery |
| 基金项目: |
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| 中文摘要: |
| 采用6种不同孔径和亲疏水性的聚苯乙烯微球，通过吸附法固定化脂肪酶YS2071，筛选得到最佳载体，对固定化条件进行了优化并对其性能进行了研究。结果表明：粒径范围0.6~1 μm的氨基聚苯乙烯微球B具有最佳的固定化效果，在酶质量浓度5 mg/mL、pH 10、固定化温度20?℃、振荡转速250 r/min、吸附时间6 h条件下，酶活回收率达到42.82%；与YS2071脂肪酶游离酶相比，经固定化后的载体脂肪酶的温度稳定性、金属离子稳定性、有机溶剂稳定性、pH稳定性均明显增强；25?℃存储30 d后，固定化酶酶活保留率比游离酶酶活保留率提高了15.09个百分点；固定化酶重复使用5 次后，酶活保留率为63.77%。 |
| 英文摘要: |
| Six kinds of polystyrene microspheres with different pore sizes and hydrophobicities were used to immobilize lipase YS2071 by adsorption, and the optimal carrier was obtained. The immobilization conditions were optimized and the properties of immobilized lipase were studied. The results showed that the amino polystyrene microspheres B with particle size in the range of 0.6-1 μm had the best immobilization effect. Under the conditions of enzyme mass concentration 5 mg/mL, pH 10, immobilization temperature 20?℃, rotational speed 250 r/min and adsorption time 6 h, the enzyme recovery was up to 4282%. Compared with YS2071 lipase free enzyme, the immobilized lipase had better temperature stability, metal ion stability, organic solvent stability and pH stability. After 30 d storage at 25?℃, the retention rate of immobilized enzyme activity was 15.09 percentage points higher than that of free enzyme. Being reused for five times, the enzyme activity retention rate of immobilized enzyme was 63.77%. |
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