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Optimization of lipase-catalyzed synthesis of tripalmitin by response surface methodology
  
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KeyWord:CalB immo Plus lipase  tripalmitin  response surface methodology  esterification
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XU Kunhua, WANG Dongming, GE Zan，etc  
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Abstract:
      Based on single factor experiment, the synthesis process of tripalmitin (PPP) from palmitic acid and glycerol by CalB immo Plus lipase catalysis in a solvent free system was optimized by response surface methodology. The results showed that the optimal synthesis process conditions were obtained as follows: reaction time 12.8 h, molar ratio of palmitic acid to glycerol 2.9∶?1, reaction temperature 75?℃ and enzyme dosage 4.4%.Under these conditions, the esterification rate and the triglycerol content were 95.44% and 85.75%, respectively.
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