响应面优化脂肪酶催化合成三棕榈酸甘油酯
Optimization of lipase-catalyzed synthesis of tripalmitin by response surface methodology
  
DOI:
中文关键词:  脂肪酶CalB immo Plus  三棕榈酸甘油酯  响应面法  酯化
英文关键词:CalB immo Plus lipase  tripalmitin  response surface methodology  esterification
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Author NameAffiliation
XU Kunhua, WANG Dongming, GE Zan,etc  
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中文摘要:
      以单因素实验为基础,采用响应面法优化脂肪酶CalB immo Plus在无溶剂体系中催化棕榈酸和甘油酯化反应合成三棕榈酯甘油酯工艺。结果表明:最佳合成工艺条件为反应时间12.8 h、底物摩尔比2.9∶?1、反应温度75?℃、加酶量4.4%,在此条件下,酯化率和甘三酯含量分别为95.44%和85.75%。
英文摘要:
      Based on single factor experiment, the synthesis process of tripalmitin (PPP) from palmitic acid and glycerol by CalB immo Plus lipase catalysis in a solvent free system was optimized by response surface methodology. The results showed that the optimal synthesis process conditions were obtained as follows: reaction time 12.8 h, molar ratio of palmitic acid to glycerol 2.9∶?1, reaction temperature 75?℃ and enzyme dosage 4.4%.Under these conditions, the esterification rate and the triglycerol content were 95.44% and 85.75%, respectively.
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