A method for determination of benzene (a) pyrene content in vegetable oil by high performance liquid chromatography was established using sample pretreatment of saponification and chromatographic elution. The results showed that the standard addition recovery and RSD of this method were 99.5%-106% and 0.68%-3.6% respectively, and pretreatment method had the advantages of low cost (less than ¥10 per sample),short time (60 min), non-toxic and environmental protection (ethanol), which was applicable to large quantities of vegetable oil in the determination of benzo(a) pyrene content. |