| Using broccoli seed as raw material, supercritical CO2 was used to extract broccoli seed oil. The effects of extraction pressure, extraction temperature, CO2 flow rate, extraction time and grinding particle size on the yield of broccoli seed oil were investigated by single factor experiment. Based on this, the supercritical CO2 extraction conditions of broccoli seed oil were optimized and determined by orthogonal experiment. The fatty acid composition of broccoli seed oil was analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the optimal extraction conditions of broccoli seed oil were obtained as follows: grinding particle size 40 meshes, extraction pressure 30 MPa, extraction temperature 50?℃, extraction time 4 h and CO2 flow rate 7 BV/h. Under these conditions, the yield of broccoli seed oil was 24.03%. The main fatty acids of broccoli seed oil were erucic acid (58.26%), oleic acid (23.76%), linoleic acid (8.99%) and palmitic acid (3.56%), in which the content of unsaturated fatty acids reached 92.36%. |
