In order to ensure the quality of Akebia trifoliate seed oil extracted by supercritical CO2, microwave technology was used to assist the pretreatment of raw materials. The conditions of microwave pretreatment and supercritical CO2 extraction were optimized by single factor experiment and orthogonal experiment. The results showed that the optimal microwave pretreatment conditions were obtained as follows: microwave pretreatment time 90 s, Akebia trifoliate seed granularity 80 meshes and Akebia trifoliate seed moisture content 7.0%. The optimal supercritical CO2 extraction conditions were obtained as follows: extraction pressure 30 MPa, extraction temperature 45?℃ and extraction time 2.5 h.Under the optimal conditions, the oil extraction rate was 95.3%,and the quality of Akebia trifoliate seed oil was higher, and total flavonoids content was up to 137.3 mg/kg. |