| The refining process and quality differences of Chinese giant salamander oils extracted by aqueous enzymatic method and hot melt method were studied. Chinese giant salamander oil was prepared respectively by flavor protease enzymatic method and hot melt method, then degummed by 0.2% of phosphate with mass fraction 80%, deacidified by 1.5% of NaOH with mass fraction 6.67%, bleached by 4% of compound decolorant, deodorized by volume ratio of material to tea polyphenol-yeast complex solution (mass fraction 2%) 1∶?3, and deodorized for 1 h at 115?℃ under vacum degree 0.08 MPa,and the refined oils were obtained. The physicochemical properties and fatty acid compositions of the two refined Chinese giant salamander oils were determined. The results showed that the extraction rates of aqueous enzyme oil and hot melt oil were 75.85% and 75.54% respectively, and the bleaching rates were 4283% and 33.61% respectively,and the fishy smell of aqueous enzyme oil deodorized by tea polyphenol-yeast complex solution was lower than that of hot melt oil. The refined aqueous enzyme oil met the first grade standard of refined fish oil in Chinese aquatic industry, and the hot melt oil met the secondary standard of refined fish oil in the aquatic industry. The contents of unsaturated fatty acid, EPA and DHA in the aqueous enzyme oil was 76.8%, 3.5% and 4.2%, respectively,which were higher than those of hot melt oil. The quality of Chinese giant salamander oil extracted by aqueous enzymatic method was better than that by hot melt method. |
