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AB-8大孔吸附树脂纯化蛋黄卵磷脂的工艺研究
Purification process of egg yolk lecithin with AB-8 macroporous adsorption resin
  
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中文关键词:  卵磷脂  分离纯化  大孔吸附树脂  中压制备色谱
英文关键词:lecithin  separation and purification  microporous adsorption resin  medium pressure preparation chromatography
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Author NameAffiliation
WU Hao, ZHOU Xuan, ZHANG Chun，etc  
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中文摘要:
      采用柱层析法对蛋黄卵磷脂纯化工艺进行研究。结果表明，从3种吸附介质中筛选出AB-8大孔吸附树脂为蛋黄卵磷脂的纯化树脂，并通过静态试验得到其对蛋黄卵磷脂的最大吸附率为922%，最佳静态吸附时间是60 min。经过中压制备色谱分离，采用干法上样，用体积分数95%的乙醇洗脱，可得到蛋黄卵磷脂的纯化倍数为4倍，纯度达到83.1%，回收率为97.04%。AB-8大孔吸附树脂使用50个操作周期时，卵磷脂吸附率仍在80%以上。表明AB-8大孔吸附树脂纯化蛋黄卵磷脂具有纯化倍数高、回收率高、时间短、重复性好等优点。
英文摘要:
      The purification process of egg yolk lecithin by column chromatography was studied. The results showed that AB-8 macroporous adsorption resin was selected from three kinds of adsorbents to purify egg yolk lecithin, the maximum adsorption rate was 92.2%, and the optimal static adsorption time was 60 min by static test. After separation by medium pressure preparation chromatography, using dry sample, eluted with 95% ethanol, purification multiple, purity and recovery rate of egg yolk lecithin were four times, 83.1% and 97.04%, respectively. The lecithin adsorption rate was above 80% when AB-8 macroporous adsorption resin was used for 50 cycles of operation. Purification process of egg yolk lecithin with AB-8 macroporous adsorption resin had advantages of high purification multiple, high recovery rate, short time, and good repeatability.
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