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| 蛋黄来源磷脂酰乙醇胺的富集、纯化及脂肪酸组成研究 |
| Enrichment,purification and fatty acid composition of phosphatidyl ethanolamine from egg yolk |
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| DOI： |
| 中文关键词: 蛋黄粉 磷脂酰乙醇胺 富集 纯化 脂肪酸组成 |
| 英文关键词:egg yolk powder phosphatidyl ethanolamine enrichment purification fatty acid composition |
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| 中文摘要: |
| 对蛋黄粉进行磷脂酰乙醇胺（PE）富集、分离纯化，采用单因素实验考察富集环节中丙酮-无水乙醇配比、溶剂倍量、洗脱时间、洗脱次数对富集PE含量的影响，并用正交实验进行优化；采用气相色谱分析了纯化蛋黄磷脂酰乙醇胺的脂肪酸组成。结果表明：PE富集的最优方案为溶剂丙酮-无水乙醇配比3∶?1、溶剂倍量2倍、洗脱时间1 h、洗脱2次，富集后PE含量达到52%，富集率为70%；经硅胶柱层分离纯化后，PE纯度达99%；蛋黄磷脂酰乙醇胺骨架含有7种脂肪酸，不饱和脂肪酸含量为55.52%，其中花生四烯酸含量达16.40%。 |
| 英文摘要: |
| The phosphatidyl ethanolamine(PE) from egg yolk powders was enriched and then separated and purified. The effects of the ratio of acetone to absolute ethanol, solvent amount, elution time and elution times on the PE content after enrichment were studied by single factor experiment, and optimized by orthogonal experiment. The fatty acid composition of purified phosphatidyl ethanolamine from egg yolk was analyzed by gas chromatography. The results showed that the optimal enrichment conditions were obtained as follows: ratio of acetone to absolute ethanol 3∶?1, solvent amount two fold, elution time 1 h and elution times twice. Under these conditions, the PE content reached 52%, and the enrichment rate was 70%. The PE purity reached 99% after purification by silica column chromatography. The egg yolk phosphatidyl ethanolamine skeleton contained seven kinds of fatty acids, and the content of unsaturated fatty acid was 55.52%, among which arachidonic acid content was up to 16.40%. |
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