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| Enrichment,purification and fatty acid composition of phosphatidyl ethanolamine from egg yolk |
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| DOI： |
| KeyWord:egg yolk powder phosphatidyl ethanolamine enrichment purification fatty acid composition |
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| Abstract: |
| The phosphatidyl ethanolamine(PE) from egg yolk powders was enriched and then separated and purified. The effects of the ratio of acetone to absolute ethanol, solvent amount, elution time and elution times on the PE content after enrichment were studied by single factor experiment, and optimized by orthogonal experiment. The fatty acid composition of purified phosphatidyl ethanolamine from egg yolk was analyzed by gas chromatography. The results showed that the optimal enrichment conditions were obtained as follows: ratio of acetone to absolute ethanol 3∶?1, solvent amount two fold, elution time 1 h and elution times twice. Under these conditions, the PE content reached 52%, and the enrichment rate was 70%. The PE purity reached 99% after purification by silica column chromatography. The egg yolk phosphatidyl ethanolamine skeleton contained seven kinds of fatty acids, and the content of unsaturated fatty acid was 55.52%, among which arachidonic acid content was up to 16.40%. |
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