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| 雨生红球藻中虾青素含量快速测定方法 |
| A rapid determination method for astaxanthin content in Haematococcus pluvialis |
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| DOI： |
| 中文关键词: 雨生红球藻 虾青素 快速测定 分光光度法 |
| 英文关键词:Haematococcus pluvialis astaxanthin rapid determination spectrophotometry |
| 基金项目: |
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| 中文摘要: |
| 雨生红球藻中虾青素以虾青素单酯、二酯以及少量游离虾青素的形式存在，为了准确测定虾青素含量，通常需要将提取的虾青素酯水解转化为游离虾青素，再利用HPLC进行定量，操作耗时，不利于生产过程的快速监测。基于系统研究分光光度法直接测定细胞提取物中的混合虾青素含量和提取-酶解-HPLC法测定的关系，发现分光光度法估算的虾青素含量与HPLC法测定的准确含量之间具有良好的线性关系（r2=0.997）。基于此建立了雨生红球藻虾青素快速测定方法，并对提取条件进行了优化。雨生红球藻粉（约5 mg）利用1 mL二甲基亚砜和6 mL丙酮进行1次提取，准确定容后，测定474 nm处的吸光度，根据吸光度与HPLC法虾青素含量间线性关系计算雨生红球藻中虾青素的含量。该方法操作简单，仅需10~20 min，测定准确，适于生产和流通环节的所需要的快速测定领域。 |
| 英文摘要: |
| Astaxanthin existed in the forms of astaxanthin esters and a small amount of free astaxanthin in Haematococcus pluvialis. The astaxanthin esters should be hydrolyzed to free astaxanthin before quantitation by HPLC, which was time consuming process and not conducive to the rapid monitoring of the production process. The relationship of determination of astaxanthin content direct by spectrophotometry and extraction-enzymatic hydrolysis-HPLC method was studied systematically. It was found that there was a good linear relationship between astaxanthin content estimated by spectrophotometry and determined by HPLC (r2=0.997). A rapid determination method for astaxanthin content in Haematococcus pluvialis was established, and the extraction conditions were optimized. The powder of Haematococcus pluvialis (about 5 mg) was extracted by 1 mL dimethylsulfoxide (DMSO) and 6 mL acetone. After constant volume, it was determined at 474 nm by spectrophotometry, and the astaxanthin content was calculated according to the linear relationship between absorption and astaxanthin content determined by HPLC. The method had advantages of simple operation and accurate measurement, which only needed 10-20 min and only used spectrophotometry. The method was suitable for rapid determination in the production and circulation links. |
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