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| A rapid determination method for astaxanthin content in Haematococcus pluvialis |
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| KeyWord:Haematococcus pluvialis astaxanthin rapid determination spectrophotometry |
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| Abstract: |
| Astaxanthin existed in the forms of astaxanthin esters and a small amount of free astaxanthin in Haematococcus pluvialis. The astaxanthin esters should be hydrolyzed to free astaxanthin before quantitation by HPLC, which was time consuming process and not conducive to the rapid monitoring of the production process. The relationship of determination of astaxanthin content direct by spectrophotometry and extraction-enzymatic hydrolysis-HPLC method was studied systematically. It was found that there was a good linear relationship between astaxanthin content estimated by spectrophotometry and determined by HPLC (r2=0.997). A rapid determination method for astaxanthin content in Haematococcus pluvialis was established, and the extraction conditions were optimized. The powder of Haematococcus pluvialis (about 5 mg) was extracted by 1 mL dimethylsulfoxide (DMSO) and 6 mL acetone. After constant volume, it was determined at 474 nm by spectrophotometry, and the astaxanthin content was calculated according to the linear relationship between absorption and astaxanthin content determined by HPLC. The method had advantages of simple operation and accurate measurement, which only needed 10-20 min and only used spectrophotometry. The method was suitable for rapid determination in the production and circulation links. |
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