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| 反胶束体系中脂肪酶催化合成单月桂酸甘油酯 |
| Enzymatic synthesis of monolaurin in reversed micellar system |
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| DOI： |
| 中文关键词: 反胶束体系 单月桂酸甘油酯 酶催化 奶酪香精脂肪酶 |
| 英文关键词:reversed micellar system monolaurin enzymatic catalysis Palatase 20000L |
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| 中文摘要: |
| 研究了在二-（2-乙基己基）琥珀酸酯磺酸钠（AOT）/异辛烷反胶束体系中，奶酪香精脂肪酶催化三月桂酸甘油酯和甘油反应制备单月桂酸甘油酯，并研究了反胶束体系的基本特性。结果表明：随着AOT质量浓度的增加，反胶束体系的增溶水量（W0）、“水池”直径及黏度增加；最佳工艺条件为反应温度55?℃、振荡器转速180 r/min、AOT质量浓度0.1 g/mL、含水量0.05 mL/mL、脂肪酶用量50 mg/mL、缓冲液pH 7.5、底物摩尔比（甘油与三月桂酸甘油酯摩尔比） 3∶?1、反应时间10 h，此时体系中单月桂酸甘油酯的质量分数为84.4%；当AOT质量浓度和含水量较小时，反胶束体系的W0增加可促进酶催化反应的进行，但是当W0和体系相对黏度继续增加，单月桂酸甘油酯的质量分数反而降低。 |
| 英文摘要: |
| Palatase 20000L was used in bis(2-ethylhexyl) sulfosuccinate (AOT)/isooctane reversed micellar system to produce monolaurin from trilaurin and glycerol, and the basic properties of reversed micellar system were explored. The results showed that with the increase of AOT mass concentration, the volume of water which could be solubilized in the system increased, as well as the viscosity and the diameter of ‘water pool’. The optimal parameters were obtained as follows:reaction temperature 55?℃, stirring rate 180 r/min, AOT mass concentration 0.1 g/mL, water content 0.05 mL/mL, lipase dosage 50 mg/mL, buffer pH 7.5, molar ratio of glycerol to trilaurin 3∶?1 and reaction time 10 h. Under these conditions, the mass fraction of monolaurin in the reaction system was 84.4%.When the AOT mass concentration and the water content were lower, the enzymatic reaction could be promoted with the increase of W0 of the reversed micellar system. However, the mass fraction of monolaurin decreased when the W0 and relative viscosity continued to increase. |
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