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| Enzymatic synthesis of monolaurin in reversed micellar system |
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| KeyWord:reversed micellar system monolaurin enzymatic catalysis Palatase 20000L |
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| Abstract: |
| Palatase 20000L was used in bis(2-ethylhexyl) sulfosuccinate (AOT)/isooctane reversed micellar system to produce monolaurin from trilaurin and glycerol, and the basic properties of reversed micellar system were explored. The results showed that with the increase of AOT mass concentration, the volume of water which could be solubilized in the system increased, as well as the viscosity and the diameter of ‘water pool’. The optimal parameters were obtained as follows:reaction temperature 55?℃, stirring rate 180 r/min, AOT mass concentration 0.1 g/mL, water content 0.05 mL/mL, lipase dosage 50 mg/mL, buffer pH 7.5, molar ratio of glycerol to trilaurin 3∶?1 and reaction time 10 h. Under these conditions, the mass fraction of monolaurin in the reaction system was 84.4%.When the AOT mass concentration and the water content were lower, the enzymatic reaction could be promoted with the increase of W0 of the reversed micellar system. However, the mass fraction of monolaurin decreased when the W0 and relative viscosity continued to increase. |
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