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| Protective effect of selenium rich oil-tea camellia seed oil on HaCaT cell injuried by hydrogen peroxide |
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| DOI： |
| KeyWord:oil-tea camellia seed oil H2O2 induction HaCaT cell injury protective effect |
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| Abstract: |
| The protective effect of oil-tea camellia seed oil on the human immortalized epidermal cells (HaCaT) injuried by hydrogen peroxide was studied. The oil-tea camellia seed oil from Camellia oleifera obtained by physical pressing was used as material. HaCaT cells cultured in vitro were used as the experimental objects. The experiment was divided into control group, H2O2 model damage group, positive control group (VC), and treatment group (oil-tea camellia seed oil, 5-500 μg/mL), using cell survival rate（apoptosis rate） in cell, SOD level, GSH-PX level, ROS level and Nrf2 gene expression as evaluation indexes to evaluate the antioxidant activity. The results showed that when oil-tea camellia seed oil was added to pre-protect the cells, the survival rate of damaged cells was 70%-82% for 50-200 μg/mL of oil-tea camellia seed oil, higher than H2O2 model group. The apoptosis rate of 50 μg/mL oil-tea camellia seed oil treatment group was 4.49%, reducing by 4477% compared with H2O2 model damage group. Compared with H2O2 model damage group, the levels of intracellular SOD (22.09 U/mg) and GSH-PX (64.50 U/mg) increased by 46.68% and 6366% respectively, with significant differences (P<005). While the level of ROS decreased significantly, the mean fluorescence intensity in cells decreased by 37.66% compared with H2O2 model damage group (88.27). At the same time, the relative expression of Nrf2 gene was 1.13, increasing by 10178% compared with H2O2 model damage group. All indexes were better than those of positive control 50 μg/mL VC treatment group. It indicated that oil-tea camellia seed oil had a certain pre-protective effect on oxidative damage caused by H2O2, better than the same mass concentration of VC. It could be further developed into a natural and effective antioxidant product. |
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