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| 利用抗性筛选和基因组重排技术选育ε-聚赖氨酸高产菌株 |
| Improving ε-poly-L-lysine productivity in Streptomyces albulus M-Z18 through antibiotic resistance screening and genome shuffling technology |
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| DOI： |
| 中文关键词: 多肽 ε-聚赖氨酸 基因组重排 抗性筛选 巴龙霉素 |
| 英文关键词:polypeptide ε-poly-L-lysine genome shuffling resistance screening paromomycin |
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| 中文摘要: |
| ε-聚赖氨酸产生菌Streptomyces albulus M-Z18的摇瓶产量较低,仅为1.60 g/L。利用抗性筛选和基因组重排技术对S. albulus M-Z18进行菌种选育以获得高产ε-聚赖氨酸菌株。通过引入巴龙霉素抗性到S. albulus M-Z18中，获得两株性状优良的菌株S. albulus P-1和S. albulus P-2，ε-聚赖氨酸产量分别为2.20 g/L和2.16 g/L，单位菌体ε-聚赖氨酸合成能力分别为0.41 g/g和0.39 g/g，作为基因组重排的亲本菌株；运用正交实验优化实验条件，最终获得一株高产ε-聚赖氨酸的融合子S. albulus G12，产量为2.73 g/L，相比M-Z18提高了70.63%。 |
| 英文摘要: |
| The ε-poly-L-lysine （ε-PL） producing strain Streptomyces albulus M-Z18 produced only 1.60 g/L in shake flasks. S. albulus M-Z18 was bred by resistance screening and genome shuffling technology to get the ε-PL high-productivity strain. The paromomycin resistance was introduced into S. albulus M-Z18, and two strains with excellent traits, S. albulus P-1 and S. albulus P-2, were obtained with ε-PL production of 2.20 g/L and 2.16 g/L, respectively. The ε-PL biosynthetic capacity per strain was 0.41 g/g and 0.39 g/g, respectively. Using S. albulus P-1 and S. albulus P-2 as the parent strains for genome shuffling, the high-yield fusions were screened using protoplast fusion technology, and finally a fusion with high production of ε-PL was obtained. The ε-PL production of S. albulus G12 was 2.73 g/L, an increase of 70.63% compared with M-Z18. |
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