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Improving ε-poly-L-lysine productivity in Streptomyces albulus M-Z18 through antibiotic resistance screening and genome shuffling technology
  
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KeyWord:polypeptide  ε-poly-L-lysine  genome shuffling  resistance screening  paromomycin
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ZHAO Junjie，WANG Kaifang，CHEN Xusheng，etc  
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Abstract:
      The ε-poly-L-lysine （ε-PL） producing strain Streptomyces albulus M-Z18 produced only 1.60 g/L in shake flasks. S. albulus M-Z18 was bred by resistance screening and genome shuffling technology to get the ε-PL high-productivity strain. The paromomycin resistance was introduced into S. albulus M-Z18, and two strains with excellent traits, S. albulus P-1 and S. albulus P-2, were obtained with ε-PL production of 2.20 g/L and 2.16 g/L, respectively. The ε-PL biosynthetic capacity per strain was 0.41 g/g and 0.39 g/g, respectively. Using S. albulus P-1 and S. albulus P-2 as the parent strains for genome shuffling, the high-yield fusions were screened using protoplast fusion technology, and finally a fusion with high production of ε-PL was obtained. The ε-PL production of S. albulus G12 was 2.73 g/L, an increase of 70.63% compared with M-Z18.
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