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| 基于微流控芯片的牡丹籽粕低聚茋类化合物抗癌活性的研究 |
| Anticancer activity of oligostilbenes from peony seed meal based on microfluidic chip |
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| DOI： |
| 中文关键词: 油用牡丹 低聚茋类化合物 微流控芯片 浓度梯度 细胞活性 |
| 英文关键词:peony for oil oligostilbenes microfluidic chip concentration gradient cell viability |
| 基金项目: |
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| 中文摘要: |
| 为探究油用牡丹籽粕低聚茋类化合物对人源性乳腺癌细胞(MCF-7）活性的影响，通过设计制作一种浓度梯度微流控芯片，利用AO/PI双染鉴定细胞在不同质量浓度低聚茋类化合物诱导下的活性情况。结果表明：细胞在药液（低聚茋类化合物）诱导下，呈明显皱缩和裂解；在药液质量浓度梯度作用下，芯片每个培养腔内细胞活力不同；进行细胞计数可知，药液质量浓度为0.5 mg/mL时，细胞活力约50%；药液质量浓度为0.75 mg/mL时，细胞活力16.17%；药液质量浓度达到 1 mg/mL时，培养腔内几乎无正常细胞。该方法实现了对药液质量浓度梯度影响细胞活性的实时监测和观察，为微流控芯片技术与油用牡丹副产物抗癌活性研究的结合提供了一种新思路和新方法。 |
| 英文摘要: |
| In order to study the inhibitory effects of oligostilbenes from peony seed meal on activity of human breast cancer cell MCF-7, by designing a novel concentration gradient microfluidic chip, AO/PI staining protocol was used to identify the activity of cells induced by different mass concentrations of oligostilbenes. The results showed that MCF-7 cells obviously crinkled and cracked under the induction of oligostilbenes. Under the action of oligostilbenes mass concentration gradient, the cells in each culture of the chip showed different activities. Cell count showed that when the mass concentration of oligostilbenes was 0.5 mg/mL, the cell viability was about 50%. When the mass concentration was 0.75 mg/mL, the cell viability was 16.17%, and when the mass concentration reached 1 mg/mL, there was almost no normal cell in the culture chamber. The method realized real-time monitoring and observation of cell activity induced by drug mass concentration gradient and provided a new way of thinking and new method for the combination of microfluidic chip technology and anticancer activity of peony for oil byproducts. |
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