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| Anticancer activity of oligostilbenes from peony seed meal based on microfluidic chip |
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| KeyWord:peony for oil oligostilbenes microfluidic chip concentration gradient cell viability |
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| Abstract: |
| In order to study the inhibitory effects of oligostilbenes from peony seed meal on activity of human breast cancer cell MCF-7, by designing a novel concentration gradient microfluidic chip, AO/PI staining protocol was used to identify the activity of cells induced by different mass concentrations of oligostilbenes. The results showed that MCF-7 cells obviously crinkled and cracked under the induction of oligostilbenes. Under the action of oligostilbenes mass concentration gradient, the cells in each culture of the chip showed different activities. Cell count showed that when the mass concentration of oligostilbenes was 0.5 mg/mL, the cell viability was about 50%. When the mass concentration was 0.75 mg/mL, the cell viability was 16.17%, and when the mass concentration reached 1 mg/mL, there was almost no normal cell in the culture chamber. The method realized real-time monitoring and observation of cell activity induced by drug mass concentration gradient and provided a new way of thinking and new method for the combination of microfluidic chip technology and anticancer activity of peony for oil byproducts. |
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