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| 栀子油对ConA诱导的小鼠急性免疫性肝损伤的保护作用 |
| Protective effect of Gardenia oil on acute immunologic liver injury induced by ConA in mice |
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| DOI： |
| 中文关键词: 栀子油 ConA 肝损伤 保肝 |
| 英文关键词:Gardenia oil ConA liver injury hepatoprotective |
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| 中文摘要: |
| 研究栀子油对刀豆蛋白A(ConA)诱导的小鼠免疫性肝损伤的保护作用，并探讨其作用机制。将小鼠随机分成空白组、模型组、联苯双酯组，栀子油低、中、高剂量组，各给药组每天给药1次，连续给药10 d，末次给药1 h后，除空白组外，其他各组尾静脉注射ConA（15 mg/kg），制备小鼠急性免疫性肝损伤模型。测定小鼠血清中ALT、AST和LDH的含量及肝脏组织匀浆中SOD活性和NO、MDA的含量，RT-PCR检测肝组织中TNF-α、CXCL-10 mRNA和IFN-γ mRNA的表达量，HE染色观察肝组织的病理学变化。结果表明：与模型组相比，栀子油各剂量组均能降低血清ALT、AST和LDH的含量，提高SOD活性，降低肝组织NO、MDA的产生，下调TNF-α、CXCL-10 mRNA和IFN-γ mRNA的相对表达量，肝组织变性及坏死等病理症状明显改善，尤以栀子油高剂量组效果最佳。栀子油对ConA诱导小鼠免疫性肝损伤有明显的保护作用，其保肝的机制可能与提高抗氧化酶体系活力，抑制氧化应激反应及炎性因子表达有关。 |
| 英文摘要: |
| The protective effect of Gardenia oil on acute immunologic liver injury induced by concanavalin A(ConA) in mice was investigated and the action mechanism was discussed.Mice were randomly divided into blank, model, biphenyl dimethylester, Gardenia oil low, middle and high dose groups. The medicine was performed once daily for 10 d, then the model of immunologic liver injury was prepared by intravenous injection of ConA(15 mg/kg) in the tail of mice in each group except for blank group one hour after the last treatment. The contents of ALT, AST and LDH in the blood serum, the activity of SOD and the levels of MDA and NO in the liver organs of mice were determined, the expressions of TNF-α mRNA, CXCL-10 mRNA and IFN-γ mRNA expressions in liver tissue were detected by RT-PCR, and pathological morphological changes in liver tissue were monitored by HE dyeing. The results showed that compared with model group, each dose group of Gardenia oil could reduce the contents of serum ALT, AST and LDH, improve the activity of SOD, reduce the productions of NO and MDA in liver tissue, and decrease the relative expressions of TNF-α mRNA, CXCL-10 mRNA and IFN-γ mRNA in liver tissue, also pathological damage in liver was relieved, meanwhile the effect of the high dose group of Gardenia oil was the best. Gardenia oil had markedly protective effect on immunologic injury induced by ConA in mice, and the mechanism might be depended on elevating antioxidant enzymes activity, inhibiting oxidative stress and inflammation factors expression. |
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