Optimization of composite enzymes ratio in bio-enzymatic extraction of peanut oil body by mixture design
  
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KeyWord:mixture design  peanut oil body  bio-enzymatic method  composite enzymes ratio
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WEI Songli, ZHANG Lixia, LU Xin,etc  
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Abstract:
      With the extraction rate of crude peanut oil body as investigation index, the composite enzymes(cellulase, pectinase, xylanase) ratio in bio-enzymatic extraction of peanut oil body was optimized by mixture design. The results showed that the effect of cellulase compound ratio on the crude peanut oil body extraction was the greatest, followed by pectinase compound ratio and xylanase compound ratio. The optimal compound ratio of cellulase to pectinase and xylanase was 0.63∶?0.24∶?0.13. Under the optimal ratio, the extraction rate of crude peanut oil body reached 47.08%±0.13%. The mixture design for the optimization of composite enzymes ratio was practical and repeatable. Besides, the extracted peanut oil body had higher purity, lighter color and better processability, which had highly potential application in industries.
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