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| Optimization of protein alkali extraction technology from Litsea cubeba and its functional properties |
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| KeyWord:Litsea cubeba protein alkaline extraction and acid precipitation functional property |
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| Abstract: |
| Litsea cubeba protein was extracted by alkali extraction and acid precipitation method from meal after oil extraction. The extraction pH, solid-liquid ratio, extraction temperature and extraction time were chosen as the factors, and the single factor experiment and orthogonal experiment were used to determine the optimal conditions. The functional properties including foaming, emulsifying and water holding capacity were evaluated. The results showed that the optimal extraction conditions were abtained as follows: extraction pH 11, solid-liquid ratio 1∶?15, extraction temperature 35?℃ and extraction time 60 min. Under these conditions, the extraction rate of Litsea cubeba protein reached 31.03%. The protein content in crude protein extract was 75%. The isoelectric point of Litsea cubeba protein was around pH 40. The solubility, foaming ability and foam stability were lower at acidic pH than alkali pH condition. The water holding capacity was higher compared with commercial soybean protein isolate when the temperature was below 60?℃. |
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