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| 川明参醇提取物抗氧化性及热稳定性研究 |
| Antioxidant activity and thermal stability of ethanol extract of Chuanminshen Violaceum Sheh et Shan |
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| DOI： |
| 中文关键词: 川明参 天然抗氧化剂 抗氧化活性 大豆油 乙醇提取物 |
| 英文关键词:Chuanminshen Violaceum Sheh et Shan natural antioxidant antioxidant activity soybean oil ethanol extract |
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| 中文摘要: |
| 以川明参为原料，采用80%的乙醇提取川明参抗氧化物质，应用体外抗氧化实验和加热氧化实验研究其抗氧化活性。先测定川明参醇提取物的DPPH、ABTS+自由基清除能力和铁还原能力3个抗氧化指标；再在大豆油中添加0.05%、0.1%、0.3%和0.5%的川明参醇提取物，添加0.02%的TBHQ作为对照，在180?℃下加热21 h，测定油脂的酸价、羰基价和脂肪酸组成。结果表明：添加川明参醇提取物具有显著的抗氧化效果，其中质量浓度为25 mg/mL的川明参醇提取物对DPPH自由基和ABTS+自由基清除能力分别为95.52%和74.41%；添加TBHQ对大豆油酸价增加的延缓效果最好，其次为03%川明参醇提取物；添加0.5%川明参醇提取物对大豆油羰基价增加的延缓效果最好；对大豆油C18∶?2的减少量延缓效果顺序为0.02%TBHQ>0.5%川明参醇提取物>0.1%川明参醇提取物>0.3%川明参醇提取物>无添加>0.05%川明参醇提取物；对大豆油C18∶?3的减少量延缓效果顺序为0.02%TBHQ>0.5%川明参醇提取物>0.3%川明参醇提取物>0.1%川明参醇提取物>0.05%川明参醇提取物>无添加。不同添加量的川明参醇提取物对大豆油的酸价、羰基价和脂肪酸组成均有不同的抗氧化效果。 |
| 英文摘要: |
| With Chuanminshen Violaceum Sheh et Shan (C. Violaceum) as raw material, antioxidant components were extracted by 80% ethanol, and antioxidant activity was analyzed by in vitro antioxidant and heating oxidation experiments. Firstly, three antioxidant indexes of DPPH and ABTS+ free radical scavenging abilities, iron reduction ability of the ethanol extract of C. Violaceum were determined, then 0.05%, 0.1%, 0.3% and 0.5% of the ethanol extract from C. Violaceum were added to soybean oil, respectively, and 0.02%TBHQ was used as a control. The soybean oils were heated at 180 ?℃ for 21 h, and the acid value, carbonyl value and fatty acid composition were determined. The results showed that the ethanol extract of C. Violaceum had a significant antioxidant effect, and the scavenging abilities of ethanol extract of C. Violaceum with mass concentration of 25 mg/mL to DPPH and ABTS+ free radical were 95.52% and 7441%, respectively. The addition of TBHQ had the best retardation effect on the increase of acid value of soybean oil, followed by 0.3% ethanol extract of C. Violaceum. The addition of 0.5% ethanol extract of C. Violaceum had the best retardation effect on the increase of carbonyl value of soybean oil. The addition of 0.02% TBHQ had the best retardation effect on the reduction of C18∶?2 in soybean oil, followed by 0.5% ethanol extract, 0.1% ethanol extract, 0.3% ethanol extract, no addition and 0.05% ethanol extract. The addition of 0.02% TBHQ had the best retardation effect on the reduction of C18∶?3 in soybean oil, followed by 0.5% ethanol extract, 0.3% ethanol extract, 0.1% ethanol extract and 0.05% ethanol extract, no addition. Different dosages of ethanol extract of C. Violaceum had different antioxidant effects on acid value, carbonyl value and fatty acid composition of soybean oil. |
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