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| Antioxidant activity and thermal stability of ethanol extract of Chuanminshen Violaceum Sheh et Shan |
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| KeyWord:Chuanminshen Violaceum Sheh et Shan natural antioxidant antioxidant activity soybean oil ethanol extract |
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| Abstract: |
| With Chuanminshen Violaceum Sheh et Shan (C. Violaceum) as raw material, antioxidant components were extracted by 80% ethanol, and antioxidant activity was analyzed by in vitro antioxidant and heating oxidation experiments. Firstly, three antioxidant indexes of DPPH and ABTS+ free radical scavenging abilities, iron reduction ability of the ethanol extract of C. Violaceum were determined, then 0.05%, 0.1%, 0.3% and 0.5% of the ethanol extract from C. Violaceum were added to soybean oil, respectively, and 0.02%TBHQ was used as a control. The soybean oils were heated at 180 ?℃ for 21 h, and the acid value, carbonyl value and fatty acid composition were determined. The results showed that the ethanol extract of C. Violaceum had a significant antioxidant effect, and the scavenging abilities of ethanol extract of C. Violaceum with mass concentration of 25 mg/mL to DPPH and ABTS+ free radical were 95.52% and 7441%, respectively. The addition of TBHQ had the best retardation effect on the increase of acid value of soybean oil, followed by 0.3% ethanol extract of C. Violaceum. The addition of 0.5% ethanol extract of C. Violaceum had the best retardation effect on the increase of carbonyl value of soybean oil. The addition of 0.02% TBHQ had the best retardation effect on the reduction of C18∶?2 in soybean oil, followed by 0.5% ethanol extract, 0.1% ethanol extract, 0.3% ethanol extract, no addition and 0.05% ethanol extract. The addition of 0.02% TBHQ had the best retardation effect on the reduction of C18∶?3 in soybean oil, followed by 0.5% ethanol extract, 0.3% ethanol extract, 0.1% ethanol extract and 0.05% ethanol extract, no addition. Different dosages of ethanol extract of C. Violaceum had different antioxidant effects on acid value, carbonyl value and fatty acid composition of soybean oil. |
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