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| Mutation breeding Schizochytrium strains producing high-yield DHA via atmospheric and room temperature plasmas |
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| DOI： |
| KeyWord:Schizochytrium limacinum SR21 DHA ARTP malonic acid iodoacetic acid |
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| Abstract: |
| A high-DHA-producing strain of the Schizochytrium limacinum SR21 was expected to obtain using the treatment of atmospheric and room temperature plasmas(ARTP), and the screening method of iodoacetic acid, malonic acid and compound plate. After treatment of ARTP for 15 s at 100 W power and 10 L/min air flow rate, a mutant strain named NA2 was selected using 1.2 g/L malonic acid as a screening plate. Cultured in shake flask for 120 h, the biomass, oil productivity and DHA productivity of NA2 strain were 18.11%, 14.87% and 46.12% higher than those of original strain, respectively. The DHA productivity reached 6.59 g/L. The results of subculture test showed that the mutant strain had stable hereditary character after five generations. |
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