蒜头果中3-酮酯酰-CoA合酶基因克隆与表达分析
Cloning and expression analysis of 3-ketoacyl-CoA synthase gene from Malania oleifera
  
DOI:
中文关键词:  蒜头果  3-酮酯酰-CoA合酶基因  荧光定量PCR  基因表达分析
英文关键词:Malania oleifera  KCS gene  RT-PCR  gene expression analysis
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LI Yunqin, CHEN Zhonghua, YUAN Xiaolong,etc  
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中文摘要:
      以蒜头果(Malania oleifera)为实验材料,基于转录组数据分析,采用RT-PCR方法获得蒜头果3-酮酯酰-CoA合酶(KCS)基因的cDNA序列,命名为MoKCS1,GenBank登录号为MK210592。序列分析显示MoKCS1基因cDNA全长为1 539 bp,编码512个氨基酸,属于KCS家族。序列比对分析显示MoKCS1拥有KCS家族特有的3个功能保守结构域,与榴莲(Durio zibethinus)KCS的蛋白序列同源性为80.66%;与已知超长链KCS蛋白进行系统进化分析显示,MoKCS1独立形成一个分支。荧光定量PCR分析表明,MoKCS1在蒜头果果实膨大期的表达量最高,而在叶中几乎不表达。
英文摘要:
      The Malania oleifera was used as material to clone the full length cDNA of the 3-ketoacyl-CoA synthase (KCS) gene based on the transcriptome of Malania oleifera with the method of RT-PCR technology, and named MoKCS1(GenBank ID: MK210592). The sequence analysis of MoKCS1 gene showed that the full-length cDNA of MoKCS1 gene was 1 539 bp, encoded 512 amino acids, and belonged to KCS family. The proteinic sequence alignment revealed that there was 80.66% of similarity between Malania oleifera and Durio zibethinus, and MoKCS1 had three functionally conserved domains that were unique in the KCS family. The phylogenetic analysis with known ultralong-chain KCS proteins revealed that no protein was found to be closely related to MoKCS1. The fluorescent quantitative RT-PCR certified that the expression of MoKCS1 gene in fruit of the flower within 3 months was the highest.
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