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| 蛋黄卵磷脂中两种溶血磷脂的分离与结构鉴定 |
| Isolation and structure identification of lysophospholipid from egg yolk lecithin |
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| DOI： |
| 中文关键词: 蛋黄卵磷脂 溶血磷脂酰胆碱 溶血磷脂酰乙醇胺 分离 结构鉴定 |
| 英文关键词:egg yolk lecithin lysophosphatidylcholine lysophosphatidyl ethanolamine isolation structure identification |
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| 中文摘要: |
| 通过柱层析分离法对蛋黄卵磷脂中的两种溶血磷脂（溶血磷脂酰胆碱和溶血磷脂酰乙醇胺）进行分离纯化，并采用ESI质谱、核磁共振氢谱和碳谱、气相色谱对其进行结构鉴定。结果表明：蛋黄来源的溶血磷脂酰胆碱主要由1-棕榈酰溶血磷脂酰胆碱，1-亚油酰溶血磷脂酰胆碱，1-硬脂酰溶血磷脂酰胆碱，1-油酰溶血磷脂酰胆碱4种化合物组成；溶血磷脂酰乙醇胺主要由1-棕榈酰溶血磷脂酰乙醇胺，1-硬脂酰溶血磷脂酰乙醇胺，1-油酰溶血磷脂酰乙醇胺3种化合物组成。 |
| 英文摘要: |
| Lysophospholipids from egg yolk lecithin were isolated and purified by column chromatography, and the structures of lysophospholipids were identified by electrospray ionization mass spectrometry, nuclear magnetic resonance hydrogen spectrum and carbon spectrum and gas chromatography. The results showed that lysophosphatidylcholine from egg yolk contained four components, including 1-hexadecanoyl-sn-glycerol-3-phosphorylcholine, 1-linoleoyl-sn-glycerol-3-phosphorylcholine, 1-stearoyl-sn-glycerol-3-phosphorylcholine and 1-oleoyl-sn-glycerol-3-phophorylcholine. Lysophosphatidyl ethanolamine contained three components, including 1-hexadecanoyl-sn-glycerol-3-phosphoethanolamine, 1-stearoyl-sn-glycerol-3-phosphoethanolamine and 1-oleoyl-sn-glycerol-3-phosphoethanolamine. |
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