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Optimization of enzymatic deacidification of crude rice bran oil
  
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KeyWord:rice bran oil  enzymatic deacidification  immobilized lipase CALB
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ZHAO Chenwei, WANG Yong, LI Mingqi，etc  
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Abstract:
      Crude rice bran oil generally has high acid value, and the conventional deacidification methods have the problems of high refining consumption, high pretreatment requirement, high loss of nutrients and poor quality of oil. Enzymatic deacidification can effectively avoid these problems. The enzymatic deacidification process of crude rice bran oil with immobilized lipase CALB was studied. The enzymatic deacidification conditions were optimized as follows: molar ratio of glycerol to free fatty acid 1∶?3, shear speed 25 m/s, shear time 20 min, enzyme dosage 50 g/kg, reaction temperature 70?℃, reaction time 6 h, water content in glycerol 25%, vacuum degree less than 1 000 Pa, adding nitrogen. Under the optimal conditions, the esterification rate was 92.5%.
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