| Peanut is an important resources of vegetable oil and protein, aqueous enzymatic treatment of peanut can simultaneously obtain oil and hydrolyzed protein with specific functions. To improve the purity of peanut hydrolyzed protein and remove salt, soluble sugar and other impurities in the peanut system, acid leaching process before enzymatic hydrolysis was designed. On the basis of single factor experiment, the leaching process was optimized by orthogonal experiment. The results showed that solid-liquid ratio and temperature had very significant or significant effect on the loss rate of protein and removal rate of soluble sugar. The optimal acid leaching process conditions were determined as follows: solid-liquid ratio 1∶?6, acid leaching time 30 min, pH 5 and acid leaching temperature 50?℃. Under these conditions, the removal rate of soluble sugar was (83.11±0.33)% and the loss rate of protein was(6.19±013)%. The content of sugar in peanut protein decreased significantly after acid leaching, also with a small amount of protein loss. |
