| Pinus koraiensis seeds in different developmental stages(July 8, August 10, September 6 and October 7 in 2016) were collected as raw materials, the oil contents in Pinus koraiensis seeds were tested using the method of chloroform methanol,and the fatty acid compositions of their oils were determined by GC-MS/MS to analyze the dynamic changes of oil content in Pinus koraiensis seeds in different developmental stages and fatty acid composition of its oil. The results showed that the oil content in Pinus koraiensis seed in early developmental stage was low (4.4%), then increased rapidly, and reached the highest oil content of 71% on September 6,then reduced. There were 13 kinds of fatty acids detected in the Pinus koraiensis seed oil,in which oleic acid, linoleic acid and pinolenic acid were the major unsaturated fatty acids. During seed development, the contents of oleic acid and linoleic acid appeared in an up-trend, but the peak value of pinolenic acid (16.30%) appeared on August 10, then decreased slightly. This data could provide scientific basis for harvest time selection of Pinus koraiensis seed, and had great significance for utilization of Pinus koraiensis seed. |
