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| 油料中苯并（a）芘含量的测定 |
| Determination of benzo（a）pyrene in oilseed |
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| DOI： |
| 中文关键词: 油料 苯并（a）芘 固相萃取 高效液相色谱法 |
| 英文关键词:oilseed benzo(a)pyrene solid-phase extraction high performance liquid chromatography |
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| 中文摘要: |
| 建立了固相萃取-高效液相色谱法测定油料中苯并(a)芘含量的方法。样品用正己烷提取，经苯并（a）芘分子印迹柱净化，采用Eclipse PAH色谱柱分离，经荧光检测器检测。结果表明：苯并(a)芘质量浓度在0.5~20.0 ng/mL范围内与峰面积线性关系良好，相关系数为1.000 0，方法检出限为005 μg/kg，定量限为0.18 μg/kg，加标回收率为71.9%~88.0%，相对标准偏差为1.24%~1011%。该方法简单、准确度高、重复性好，可用于油料中苯并(a)芘含量的检测。 |
| 英文摘要: |
| A method was developed for determination of benzo(a)pyrene in oilseed by solid-phase extraction coupled with high performance liquid chromatography.The sample was extracted by n-hexane,cleaned-up using benzo(a)pyrene molecularly imprinted column,then separated on an Eclipse PAH chromatographic column, and detected by fluorescence detector. The results showed that the mass concentration of benzo(a)pyrene had a good linearity in the range of 0.5-20.0 ng/mL with peak area, and the correlation coefficient was 1.000 0. The limit of detection was 0.05 μg/kg, and the limit of quantitation was 0.18 μg/kg. The recovery rate was 71.9%-88.0%, and the relative standard deviation was 1.24%-1011%.The method could be used to determine benzo(a)pyrene in oilseed due to its simple operation, high accuracy and good repeatability. |
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