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| Optimization of process of cellulase and alkaline protease for improving soybean oil yield by cold-pressing |
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| DOI： |
| KeyWord:soybean oil cellulose alkaline protease cold-pressing oil yield |
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| Abstract: |
| Soybean oil was prepared by cold-pressing technology with the oil yield as evaluation index. The process conditions of the combination of cellulase and alkaline protease for improving the cold-pressed soybean oil yield were optimized by single factor experiment and orthogonal experiment. The compound enzymatic cold-pressing conditions were determined as follows: particle size 40 meshes, moisture content 13%, cold-pressing temperature 80?℃, screw speed 36 r/min and compound enzyme addition 0.2%(volume ratio of cellulase to alkaline protease 1∶?2). Under these conditions, the oil yield was 706%, significantly higher by 2.1 percentage points than 68.5% of the cold-pressing process without enzyme treatment. The obtained soybean oil had unique aroma and flavor of soybean oil with brownish red, which was clear without impurities,and the acid value and peroxide value were 0.8 mgKOH/g and 6.1 mmol/kg respectively. |
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