| The urea adduction fractionation method was used to enrich lauric acid in coconut oil. Coconut oil was used as raw material to prepare coconut oil mixed fatty acid by saponification and acid hydrolysis, and then the lauric acid was enriched by urea adduction fractionation from mixed fatty acid. Through single factor experiment and orthogonal experiment optimization, the order of the six factors affecting the embedding effect from high to low was obtained as follows: water bath temperature, embedding time, mass ratio of urea to mixed fatty acid, water bath time, mass ratio of urea to methanol and embedding temperature. The optimal enrichment conditions of lauric acid in coconut oil by urea adduction fractionation method were obtained as follows: mass ratio of urea to methanol 1∶?2, water bath temperature 50?℃, embedding time 10 h, mass ratio of urea to mixed fatty acid 1∶?1.5, water bath time 5 h and embedding temperature -20?℃. Under these conditions, the content of lauric acid after enrichment was 60.05%, increasing by 9.35 percentage points compared with 50.70% of that before enrichment. |
